Infrared spectroscopy can detect E. coli faster than current testing methods and can cut days off investigations of outbreaks, according to a study at Purdue University.
Lisa Mauer, an associate professor of food science, detected E. coli in
ground beef in one hour using Fourier transform infrared spectroscopy, much
less than the 48 hours required for conventional plating technology, which
requires culturing cells in a laboratory. Mauer said spectroscopy could be done
in the same laboratories, just in much less time.
The spectroscopy method also differentiates between strains of E. coli
0157:H7, meaning outbreaks could be tracked more effectively and quickly.
Current tests are multistep and take almost one week to get results.
"Even with all the other bacteria present in ground beef, we could
still detect E. coli and recognize different strains," says Mauer, whose
findings were reported in the August issue of the Journal of Food Science.
Mauer demonstrated two methods for separating bacteria from ground beef for
testing. An antibody-capture method, which binds bacteria to antibodies
attached to magnetic beads, gave results in four hours. A filtration method
achieved results in about an hour.
Infrared spectroscopy could detect as little as one E. coli cell if the
bacteria was cultured for six hours. Conventional plating techniques used for
E. coli detection require culturing cells for 48 hours.
E. coli has a specific infrared spectrum that can be read with a Fourier
transform infrared spectrometer. Infrared light is passed over a sample. The
spectrometer reads the spectrum created by the combination of energy that has
been absorbed and energy that has been reflected back.
"Energy is only absorbed by certain components of a sample," Mauer
says. "If that component or bacteria isn't there, the energy is reflected
Mauer's testing methods also can differentiate between living and dead E.
coli cells, something current testing methods cannot.
"If the cells are dead, they're not harmful. But the presence of that
dead population could tell you something about the quality of the
product," Mauer says.
Mauer believes the ground beef tests show promise for using the technology
to find other pathogens in additional types of foods. She has already shown
that spectroscopy can detect melamine - which sickened about 300,000 infants in
China and killed at least six in 2008 - down to one part per million in
powdered baby formula.
Test Finds E. coli in Beef Faster, Could Better Trace Outbreaks
November 3, 2010