Researchers at the National Institute of Standards and Technology have added another innovation — miniature valves — to their ever-growing collection of inexpensive, easy-to-manufacture and highly efficient microfluidic devices made from plastic films and double-sided tape.
Traditionally, microfluidic devices — tiny gadgets with fluid-carrying channels used in "lab-on-a-chip" chemical analyzers, medical diagnostics and DNA forensics — have been fabricated like microchips using photolithography. A desired pattern of micrometer-sized channels and ports is created on top of a silicon substrate, which can then be replicated many times by techniques such as molding or embossing. The process requires specialized cleanroom equipment and can take several days to complete. If valves are needed in the system, they traditionally have been made from silicones. Unfortunately, silicones are not the best materials to use with particular laboratory assays or for manufacturing lab-on-a-chip structures.
NIST researchers have spent the past few years developing and refining a method for making microfluidic devices using plastic films and double-sided tape that produces a functional apparatus in hours and requires only simple tools to create channels and ports. The NIST designs allow for folding the films to make multilayer or 3-D structures, can be used to make devices with multiple functions, and cost a fraction of traditional fabrication techniques.
According to the researchers, until now, there has not been a practical way to incorporate valves for dynamic control of fluid flow in these devices. In a new paper in the journal Lab on a Chip, NIST bioengineer Gregory Cooksey and research engineer Javier Atencia describe the first-ever technique for building pneumatic microvalves into 2-D and 3-D microfluidic devices made with plastic films and tape.
Like previous NIST systems, the valved microfluidic device is built in layers. Narrow slits and holes are cut into pieces of double-sided tape that become tiny channels and ports when the tape is folded on itself. The microvalve is made by sandwiching a flexible membrane between two channels that intersect, one on top of the other. Applying air pressure to the top channel pushes the membrane down like a diaphragm valve, closing the lower channel.