New NIST research brings cellulosic ethanol processing one step closer to a more efficient production method. The biofuel can be made from corn harvest leftovers, reducing the need to divert food crops to energy use.

It's no secret that the processes for making ethanol from cellulosic sources are inefficient. However, researchers at the National Institute of Standards and Technology (NIST) are studying some of the most fundamental processes involved in extracting sugars from biomass, which is the first step in producing ethanol by fermentation. Their findings should help engineers improve process designs in order to extract the maximum amount of fuel from a given measure of biomass.

Most of the ethanol produced in the United States is created by fermenting the sugars and starch found in corn. The ability to convert inedible plants and agricultural waste into usable sources for ethanol production will help supplement alternatives to fossil fuels while reducing the diversion of food crops to energy use.

Glucose can be extracted from two substances found in most plants; cellulose, the long molecule chains comprising the cell walls of green plants, and its flimsier cell-wall counterpart, hemicellulose. The extracted glucose then is converted easily by fermentation to ethanol.

NIST researchers, in collaboration with the National Renewable Energy Laboratory in Golden, Colo., have defined the theoretical limits of reactions important to breaking apart cellulose and hemicellulose to produce glucose. They also determined that the energy needed to rupture these key bonds is a constant value of each molecular bond that is broken during the reactions.

According to Yadu Tewari, Brian Lang and Robert Goldberg, chemists at NIST, cellulose and hemicellulose both present problems to would-be ethanol producers. "Cellulose and hemicellulose are recalcitrant," Goldberg said. "They don't want to break down. It takes a long time for wood to rot. It even takes termites a long time to break wood down, and they're pretty good at it. Ethanol producers face the same problem. Because of the way these molecules are arranged, it's difficult to get access to the reactive centers in wood and other biomass. What we have done is to study some of the most basic reactions associated with the breakdown of these materials."

With enzymes to speed the reactions, the team used calorimetry and chromatography to measure the thermodynamic property values of several reactions associated with the breakdown of cellulosic and hemicellulosic substances. Because process design and bioengineering benefit from the availability of these values, the data obtained in this investigation represent "a small but significant step toward maximizing the efficiency of biomass utilization," Tewari said.